Evaluation of the correlation between serum lipid characteristics of obese subjects and ADIPOQ gene rs266729 polymorphism in Chaharmahal and Bakhtiari Province of Iran

Negar Rabiee, Roohollah Nakhaei Sistani, Ali Mohammad Ahadi, Reza Baharloo

Abstract


Obesity is a multifactorial disorder that is influenced by various factors such as behavior, diet, environment, metabolic and genetic. This disease is the result of an imbalance between energy absorption and expenditure. Mutations in genes that are responsible for appetite control and metabolism are considered as the genetic component of obesity. Adiponectin protein is one of the most effective adipokines in regulating the body's energy homeostasis and fat storage, which is expressed by the ADIPOQ gene and secreted from white adipose tissue. The concentration of this protein in the blood decreases in obesity. In this study, the relationship between rs266729 polymorphism in the ADIPOQ gene with the level of biochemical parameters such as total cholesterol and triglyceride and HDL and LDL in the blood of obese people in Borujen (a city in Iran) was investigated. This study was performed on 100 people who referred to the Tamin Ejtemaee clinic due to obesity problems in Borujen. In this study, the ARMS-PCR method was used to determine the genotype of individuals. Based on the results of this study, no significant relationship was found between biochemical parameters including total cholesterol, triglyceride, and LDL with rs266729 polymorphism genotypes in ADIPOQ gene in obese subjects. We concluded that rs266729 polymorphism cannot be useful as an index parameter for predispose genotype for imbalance in total cholesterol, triglyceride, and LDL levels in a person.


Keywords


Obesity, ADIPOQ gene, Cholesterol, Triglyceride

References


Albuquerque D, Stice E, Rodríguez-López R, Manco L, Nóbrega C. Current review of genetics of human obesity: from molecular mechanisms to an evolutionary perspective. Mol Genet Genomics. 2015; 290(4):1191–221.

Nigro E, Scudiero O, Monaco ML, Palmieri A, Mazzarella G, Costagliola C, et al. New insight into adiponectin role in obesity and obesity-related diseases. Biomed Res Int. 2014; 2014:658913.

Greenberg AS, Obin MS. Obesity and the role of adipose tissue in inflammation and metabolism. Am J Clin Nutr. 2006; 83(2):461–5.

Reddon H, Guéant JL, Meyre D. The importance of gene-environment interactions in human obesity. Clin Sci. 2016; 130(18):1571–97.

Cao H. Adipocytokines in obesity and metabolic disease. J Endocrinol. 2014; 220(2):T47–59.

Breitfeld J, Stumvoll M, Kovacs P. Genetics of adiponectin. Biochimie. 2012; 94(10):2157–63.

Lim S, Quon MJ, Koh KK. Modulation of adiponectin as a potential therapeutic strategy. Atherosclerosis. 2014; 233(2):721–8.

Alsaleh A, Sanders TAB, O’Dell SD. Effect of interaction between PPARG, PPARA and ADIPOQ gene variants and dietary fatty acids on plasma lipid profile and adiponectin concentration in a large intervention study. Proc Nutr Soc. 2012; 71(1):141–53.

Wanders D. Pharmacological effects of lipid-lowering drugs on circulating adipokines. World J Diabetes. 2010; 1(4):116-28.

Bouchard C. Gene-environment interactions in the etiology of obesity: Defining the fundamentals. Obesity. 2008; 16 (Suppl 3):S5–S10.

Coelho M, Oliveira T, Fernandes R. Biochemistry of adipose tissue: An endocrine organ. Arch Med Sci. 2013; 9(2):191–200.

Ntzouvani A, Fragopoulou E, Panagiotakos D, Pitsavos C, Antonopoulou S. Reduced circulating adiponectin levels are associated with the metabolic syndrome independently of obesity, lipid indices and serum insulin levels: a cross-sectional study. Lipids Health Dis. 2016; 15(1):140.

Wanders D, Plaisance EP, Judd RL. Lipid-Lowering Drugs and Circulating Adiponectin. In: Vitamins and Hormones [Internet]. 1st ed. Elsevier Inc.; 2012. p. 341–74. Available from: http://dx.doi.org/10.1016/B978-0-12-398313-8.00013-0.

Christou GA, Kiortsis DN. Adiponectin and lipoprotein metabolism. Obes Rev. 2013; 14(12):939–49.

Zhao T, Zhao J. Genetic effects of adiponectin on blood lipids and blood pressure. Clin Endocrinol (Oxf). 2011; 74(2):214–22.

Matsuzawa Y. Adiponectin: Identification, physiology and clinical relevance in metabolic and vascular disease. Atheroscler Suppl. 2005; 6(2):7–14.

Shirakawa T, Nakajima K, Yatsuzuka S, Shimomura Y, Kobayashi J, Machida T, et al. The role of circulating lipoprotein lipase and adiponectin on the particle size of remnant lipoproteins in patients with diabetes mellitus and metabolic syndrome. Clin Chim Acta. 2015; 440:123–32.

Gibas-Dorna M, Piątek J, Kupsz J, Bernatek M, Krauss H, Sowińska A, et al. Relationship between adipokines and lipid profile in postmenopausal women with different apolipoprotein E genotypes. Women Heal. 2017; 57(8):891–904.

Gradinaru D, Margina D, Borsa C, Ionescu C, Ilie M, Costache M, et al. Adiponectin: possible link between metabolic stress and oxidative stress in the elderly. Aging Clin Exp Res. 2017; 29(4):621–9.

Eslamian M, Mohammadinejad P, Aryan Z, Nakhjavani M, Esteghamati A. Positive Correlation of Serum Adiponectin with Lipid Profile in Patients with Type 2 Diabetes Mellitus is Affected by Metabolic Syndrome Status. Arch Iran Med. 2016; 19(4):269–74.

Durrani S, Shah J, Khan MA, Jan MR. Relationship of adiponectin level with lipid profile in type-2 diabetic men with coronary heart disease. J Ayub Med Coll Abbottabad. 2015; 27(1):32–5.

Katsiki N, Mantzoros C, Mikhailidis DP. Adiponectin, lipids and atherosclerosis. Curr Opin Lipidol. 2017; 28(4):347–54.

Jafari M, Pirouzi A, Anoosheh S, Farnia P, Tajik N. Rapid and Simultaneous Detection of Vitamin D Receptor Gene Polymorphisms by a Single ARMS-PCR Assay. Mol Diagn Ther. 2014; 18(1):97–103.

Huang T, Zhuge J, Zhang WW. Sensitive detection of BRAF V600E mutation by Amplification Refractory Mutation System (ARMS)-PCR. Biomark Res. 2013; 1(1):3.

Najafi M, Firoozrai M, Gohari HL, Zavarehie A, Basiri G. Direct haplotyping of bi-allelic SNPs using ARMS and RFLP analysis techniques. Biomol Eng. 2007; 24(6):609–12.

Ahlawat S, Sharma R, Maitra A, Roy M, Tantia MS. Designing, optimization and validation of tetra-primer ARMS PCR protocol for genotyping mutations in caprine Fec genes. Meta Gene. 2014; 2:439–49.

Vasseur F, Meyre D, Froguel P. Adiponectin, type 2 diabetes and the metabolic syndrome: lessons from human genetic studies. Expert Rev Mol Med. 2006; 8(27):1–12.

Ambroziak M, Kolanowska M, Bartoszewicz Z, Budaj A. Adiponectin gene variants and decreased adiponectin plasma levels are associated with the risk of myocardial infarction in young age. Gene. 2018; 642:498–504.

Chiodini BD, Specchia C, Gori F, Barlera S, D’orazio A, Pietri S, et al. Adiponectin gene polymorphisms and their effect on the risk of myocardial infarction and type 2 diabetes: An association study in an Italian population. Ther Adv Cardiovasc Dis. 2010; 4(4):223–30.

Thanasias E, Polychronakis I, Kampen V Van, Brüning T, Merget R. Respiratory Regulation - The Molecular Approach [Internet]. Pokorski M, editor. Dordrecht: Springer Netherlands; 2013. 47–53 p. (Advances in Experimental Medicine and Biology; vol. 756). Available from: http://link.springer.com/10.1007/978-94-007-4549-0.

Han TS, Lean MEJ. Metabolic syndrome. Medicine (Baltimore). 2015; 43(2):80–7.

Feingold KR, Grunfeld C. Obesity and Dyslipidemia. [Updated 2018 Apr 10]. In: Feingold KR, Anawalt B, Boyce A, et al., editors. Endotext [Internet]. South Dartmouth (MA): MDText.com, Inc.; 2000-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK305895/

Wang H, Peng DQ. New insights into the mechanism of low high-density lipoprotein cholesterol in obesity. Lipids Health Dis. 2011; 10:1–10.

Klop B, Elte JWF, Cabezas MC. Dyslipidemia in Obesity: Mechanisms and Potential Targets. Nutrients. 2013; 5(4):1218–40.

Dastani Z, Hivert MF, Timpson N, Perry JRB, Yuan X, Scott RA, et al. Novel loci for adiponectin levels and their influence on type 2 diabetes and metabolic traits: A multi-ethnic meta-analysis of 45,891 individuals. PLoS Genet. 2012; 8(3): e1002607.

Karmelić I, Lovrić J, Božina T, Ljubić H, Vogrinc Ž, Božina N, et al. Adiponectin Level and Gene Variability Are Obesity and Metabolic Syndrome Markers in a Young Population. Arch Med Res. 2012; 43(2):145–53.

Kaftan AN, Hussain MK. Association of adiponectin gene polymorphism rs266729 with type two diabetes mellitus in Iraqi population. A pilot study. Gene. 2015 Oct;570(1):95–9.

Sun H, Gong ZC, Yin JY, Liu HL, Liu YZ, Guo ZW, et al. The association of adiponectin allele 45T/G and -11377C/G polymorphisms with Type 2 diabetes and rosiglitazone response in Chinese patients. Br J Clin Pharmacol. 2008; 65(6):917–26.

Landecho MF, Tuero C, Valentí V, Bilbao I, de la Higuera M, Frühbeck G. Relevance of leptin and other adipokines in obesity-associated cardiovascular risk. Nutrients. 2019; 11(11):1–16.

Rabiee F, Lachinani L, Ghaedi S, Nasr-Esfahani MH, Megraw TL, Ghaedi K. New insights into the cellular activities of Fndc5/Irisin and its signaling pathways. Cell Biosci. 2020; 10(1):51.




DOI: https://doi.org/10.52547/JCBioR.1.2.66

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 © The Author(s)

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.