Identification of bacterial isolates from nasolacrimal duct infection in children with congenital nasolacrimal duct obstruction from Feiz teaching hospital, Isfahan

Helma Ebneali, Fereshteh Saffari, Alireza Zandi, Jamshid Faghri


Dacryocystitis is a lacrimal sac and duct inflammation. It can be inherited or congenital. Two primary forms are acquired dacryocystitis, acute and chronic. The goal of this study is to recognize common bacteria causing nasolacrimal duct infection in children with congenital nasolacrimal duct obstruction and to determine their antimicrobial susceptibility profiles. This cross-sectional research was conducted from January to February 2017 in the Department of Ophthalmology affiliated to Isfahan University of Medical Sciences (center of Iran). Using phenotypic and genotypic approaches, identification of specimens was performed. Disc diffusion method was used for checking antibiotic susceptibility. All of the 59 isolates from the culture of specimens belonged to Gram-positive cocci. Staphylococcus epidermidis was the predominant species (n= 44, 74.6%) followed by Staphylococcus aureus (n= 11, 18.6%), Staphylococcus haemolyticus (n= 2, 3.4%) and each of Staphylococcus saprophyticus, and Streptococcus pneumoniae (n= 1, 1.7%). Totally, the highest resistance was found against erythromycin and tetracycline while, chloramphenicol, and ciprofloxacin showed the highest susceptibility. The current research is useful in evaluating the suitable antibiotic in our area for the systemic treatment of dacryocystitis. The most effective agents against the most common isolates were chloramphenicol and ciprofloxacin. Since the bacteriology of nasolacrimal duct infections varies from region to region, it is recommended that further studies in other areas of our country can be detected the etiology of bacterial pathogens involved in acute infections.


Dacryocystitis; Antibiotic resistance; Congenital nasolacrimal duct obstruction; Bacteriology


Bharathi MJ, Ramakrishnan R, Maneksha V, Shivakumar C, Nithya V, Mittal S. Comparative bacteriology of acute and chronic dacryocystitis. Eye. 2008; 22(7):953-60.

Ali MJ, Joshi SD, Naik MN, Honavar SG. Clinical profile and management outcome of acute dacryocystitis: two decades of experience in a tertiary eye care center. Semin Ophthalmol. 2015; 30(2):118-23.

Ali MJ, Motukupally SR, Joshi SD, Naik MN. The microbiological profile of lacrimal abscess: two decades of experience from a tertiary eye care center. J Ophthalmic Inflamm Infect. 2013; 3(1):57.

Huber E, Steinkogler FJ, Huber-Spitzy V. A new antibiotic in the treatment of dacryocystitis. Orbit. 1991; 10(1):33-5.

Chen L, Fu T, Gu H, Jie Y, Sun Z, Jiang D, et al. Trends in dacryocystitis in China: A STROBE-compliant article. Medicine. 2018; 97(26):e11318.

Eslami F, Ghasemi Basir HR, Moradi A, Heidari Farah S. Microbiological study of dacryocystitis in northwest of Iran. Clin Ophthalmol. 2018; 12:1859-64.

Negm S, Aboelnour A, Saleh T, Yasser M, Hassanin O. Clinicobacteriological study of chronic dacryocystitis in Egypt. Bull Natl Res Cent. 2019; 43(1):35.

Chung SY, Rafailov L, Turbin RE, Langer PD. The microbiologic profile of dacryocystitis. Orbit. 2019; 38(1):72-8.

Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; 27th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2017.

Dilhari A, Sampath A, Gunasekara C, Fernando N, Weerasekara D, Sissons C, et al. Evaluation of the impact of six different DNA extraction methods for the representation of the microbial community associated with human chronic wound infections using a gel-based DNA profiling method. AMB Express. 2017; 7(1):179.

Brook I. Ocular infections due to anaerobic bacteria in children. J Pediatr Ophthalmol Strabismus. 2008; 45(2):78-84.

Chaudhry IA, Shamsi FA, Al-Rashed W. Bacteriology of chronic dacryocystitis in a tertiary eye care center. Ophthalmic Plast Reconstr Surg. 2005; 21(3):207-10.

Badhu BP, Karki BS, Khanal B, Dulal S, Das H. Microbiological patterns of chronic dacryocystitis. Ophthalmology. 2006; 113(12):2377.e1-2.

Sun X, Liang Q, Luo S, Wang Z, Li R, Jin X. Microbiological analysis of chronic dacryocystitis. Ophthalmic Physiol Opt. 2005; 25(3):261-3.

Huber-Spitzy V, Steinkogler FJ, Haselberger C. [The pathogen spectrum in neonatal dacryocystitis]. Klin Monbl Augenheilkd. 1987; 190(5):445-6.

Sainju R, Franzco AA, Shrestha MK, Ruit S. Microbiology of dacryocystitis among adults population in southern Australia. Nepal Med Coll J. 2005; 7(1):18-20.

Gerkowicz M, Kozioł-Montewka M, Pietraś-Trzpiel M, Kosior-Jarecka E, Szczepanik A, Latalska M. [Identification of bacterial flora of conjunctival sac in congenital nasolacrimal duct obstruction in children]. Klin Oczna. 2005; 107(1-3):83-5.

Assefa Y, Moges F, Endris M, Zereay B, Amare B, Bekele D, et al. Bacteriological profile and drug susceptibility patterns in dacryocystitis patients attending Gondar University Teaching Hospital, Northwest Ethiopia. BMC Ophthalmol. 2015; 15:34-41.

Mills DM, Bodman MG, Meyer DR, Morton AD, 3rd. The microbiologic spectrum of dacryocystitis: a national study of acute versus chronic infection. Ophthalmic Plast Reconstr Surg. 2007; 23(4):302-6.

Razavi ME, Ansari-Astaneh MR, Farzadnia M, Rahmaniyan H, Moghiman T. Bacteriological evaluation of adult dacryocystitis in Iran. Orbit. 2010; 29(5):286-90.

Begum NN, Al-Khattaf AS, Al-Mansouri SM, Yeboah EA, Kambal AM. A study of bacterial isolates from corneal specimens and their antibiotic resistance profile. Saudi Med J. 2006; 27(1):41-5.

Hartikainen J, Lehtonen OP, Saari KM. Bacteriology of lacrimal duct obstruction in adults. Br J Ophthalmol. 1997; 81(1):37-40.

Kebede A, Adamu Y, Bejiga A. Bacteriological study of dacryocystitis among patients attending in Menelik II Hospital, Addis Ababa, Ethiopia. Ethiop Med J. 2010; 48(1):29-33.

Prokosch V, Prokosch JE, Promesberger J, Idelevich EA, Böhm MR, Thanos S, et al. Bacterial spectrum and antimicrobial susceptibility patterns in acquired and connatal lacrimal duct stenosis. Curr Eye Res. 2014; 39(11):1069-75.

Kuchar A, Lukas J, Steinkogler FJ. Bacteriology and antibiotic therapy in congenital nasolacrimal duct obstruction. Acta Ophthalmol Scand. 2000; 78(6):694-8.

A V P, Patil SS, Koti SV, J S A, Garag SS, Hegde JS. Clinico-bacteriological study of chronic dacryocystitis cases in northern karnataka, India. J Clin Diagn Res. 2013; 7(11):2502-4.

Briscoe D, Rubowitz A, Assia E. Changing bacterial isolates and antibiotic sensitivities of purulent dacryocystitis. Orbit. 2005; 24(1):29-32.

Shah CP, Santani D. A comparative bacteriological profile and antibiogram of dacryocystitis. Nepal J Ophthalmol. 2011; 3(2):134-9.

Pinar-Sueiro S, Sota M, Lerchundi TX, Gibelalde A, Berasategui B, Vilar B, et al. Dacryocystitis: Systematic Approach to Diagnosis and Therapy. Curr Infect Dis Rep. 2012; 14:137–46.

Chen YL, Lee CC, Lin YL, Yin KM, Ho CL, Liu T. Obtaining long 16S rDNA sequences using multiple primers and its application on dioxin-containing samples. BMC Bioinformatics. 2015;16 (18):S13-9.

Depardieu F, Perichon B, Courvalin P. Detection of the van alphabet and identification of enterococci and staphylococci at the species level by multiplex PCR. J Clin Microbiol. 2004; 42(12):5857-60.

Al-Talib H, Yean CY, Al-Khateeb A, et al. A pentaplex PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus and Panton-Valentine Leucocidin. BMC Microbiol. 2009; 9:113.



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