The occurrence of antibiotic resistance, ESBLs, MBL and NDM-1 in Uropathogenic Escherichia coli in Central part of Iran

Farshad Kakian, Javad Fathi, Fatemeh Alvandi, Mahnaz Moumivand, Abbas Rabei Gholami, Abolfazl Gholipour, Mohamad Hossein Rezaei, Behnam Zamanzad


Broad-spectrum β-lactamase enzymes capable of destroying the antibiotics of β-lactam, cephalosporin, and Metallo-β-lactamase enzymes can also deactivate all β-lactams and carbapenems. This study aimed to determine ESBLs and MBLs enzymes and the frequency of NDM-1 gene­. In this study, 200 Escherichia coli isolates of women with urinary tract infection were collected (100 inpatients and 100 outpatients). Minimum inhibitory concentration (MIC) for ceftazidime and meropenem was determined by E-test. A phenotypic confirmation test was used to detect ESBL enzymes. MBLs production was performed with modified Hodge test (MHT) and EDTA disk synergy (EDS) test. PCR was used for detecting the presence of NDM-1 gene. From 200 isolates, 93 isolates produce ESBL enzymes. Overall, 97 isolates were resistant to ceftazidime, and 38 isolates resistant to meropenem. The results of the MHT and EDS positive tests were 41 and 16 isolates, respectively. NDM-1 was not found in any of the patients. The prevalence of E. coli isolates producing both ESBLs and MBLs enzymes, is a serious threat to clinical infections. Accordingly, for the control and treatment of these strains, rapid and accurate identification can be greatly helpful.


Escherichia coli; MIC; ESBL; NDM-1


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